PhD Position "NAD-modified regulatory RNA – function and metabolism" Institute for Pharmacy and Molecular Biotechnology

NAD-modified regulatory RNA – function and metabolism.


We have recently discovered that certain regulatory RNAs (sRNAs) in E. coli are covalently linked to the ubiquitous redox cofactor NAD (nicotinamide adenine dinucleotide) in a way similar to a eukaryotic cap (Cahová et al., Nature 2015 (519) 374). First experiments indicate a role of this modification for the function and for the turnover of the sRNAs. This first description of a cap-like structure in prokaryotes provides an unprecedented connection between regulation and metabolism. Within this project we want to explore the scope and biological function(s) of this new RNA modification in different bacteria. We will conduct experiments to identify the capping / decapping machinery, isolate interaction partners of NAD-RNAs, investigate the dependence of NAD capping on growth phase, stress conditions, and redox state. We will also search for the occurrence of reduced NADH-RNAs. We will also search for NAD-modified RNAs in eukaryotic samples.


This project will involve a wide variety of methods from biochemistry, molecular biology and microbiology, as well as analysis of NGS data. We are looking for a PhD student with an M. Sc. in biochemistry, molecular biotechnology or related disciplines and hands-on experience in RNA or protein biochemistry and/or microbiology.